gene pulser mxcell system (Bio-Rad)

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Bio-Rad gene pulser mxcell system
Gene Pulser Mxcell System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 5437 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gene+pulser+mxcell/Gene+Pulser+Xcell+Total+System/bio_rxiv__64898__2025__12__25__696462-215-26-30
Average 97 stars, based on 5437 article reviews

gene pulser mxcell system - by Bioz Stars, 2026-10

97/100 stars

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Flow Cytometry:

Article Title: Large-scale preparation of fluorescence multiplex host cell reactivation (FM-HCR) reporters.
Article Snippet: Repair of DNA damage is a critical survival mechanism that affects susceptibility to various human diseases and represents a key target for cancer therapy.. A major barrier to applying this knowledge in research and clinical translation has been the lack of efficient, quantitative functional assays for measuring DNA repair capacity in living primary cells.. To overcome this barrier, we recently developed a technology termed ‘fluorescence multiplex host cell reactivation’ (FMHCR).

Selection:

Imaging:

Nucleic Acid Electrophoresis:

Transfection:

Article Title: A cell-based interferon-tau assay with an interferon-stimulated gene 15 promoter.
Article Snippet: .. Transient and stable transfections were performed using an electroporator (ECM 2001; BTX Molecular Delivery Systems, Holliston, MA, USA) or Gene Pulser MXcell (Bio-Rad Laboratory, Hercules, CA, USA). .. In brief, MDBK cells were dispersed by the application of 0.25% trypsin and 50 mM EDTA solution (Sigma-Aldrich), and suspended at 2 × 107 cells/mL in HEPES-buffered saline (HBS) solution (21 mM HEPES (pH 7.05), 137 mM NaCl, 5 mM KCl, 0.7 mM Na2HPO4, 6 mM glucose) with constructs.

Electroporation:

Article Title: Electroporation.
Article Snippet: .. Newer machines like the Gene Pulser MXcell (Bio-Rad) also allow high-throughput electroporation using lower cell numbers in a plate well format (12, 24, or 96 well). ..

Article Title: Impact of Peptide Sequence on Functional siRNA Delivery and Gene Knockdown with Cyclic Amphipathic Peptide Delivery Agents
Article Snippet: .. Cav1–siRNA (Santa Cruz Biotechnology sc-29241, 15 pmol, 1.5 μL of 10 μM) for caveolin-1 or CLTC–siRNA (Origene SR300867, 15 pmol, 1.5 μL of 10 μM) for Clathrin heavy chain was added, and these suspensions were then transferred to 0.2 cm electroporation cuvettes before a square-wave 250 V, 2000 μF, 1000 Ω, 20 ms, single pulse was applied using a Bio-Rad Gene Pulser MXCell. .. Cells were then recovered in DMEM containing 10% FBS plated at 5 × 10 5 cells/well in a six-well plate for 24 h before uptake experiments and/or protein extraction by Reporter Lysis Buffer (Promega E397A) per manufacturer recommendations.

Plasmid Preparation:

Article Title: A Domesticated PiggyBac Transposase Interacts with Heterochromatin and Catalyzes Reproducible DNA Elimination in Tetrahymena
Article Snippet: .. The cells were washed in 10 mM HEPES pH 7.5 and resuspended in 120 μl of 10 mM HEPES and then mixed with 120 μl of plasmid DNA in 10 mM HEPES (300 ng/μl) and electroporated (220 V, 50 Ω, 50 μF, exponential pulse) using a BioRad Gene Pulser MXcell. ..

other:

Article Title: β-arrestin 2 Is a Prognostic Factor for Survival of Ovarian Cancer Patients Upregulating Cell Proliferation
Article Snippet: Briefly, 3.5 × 10 4 cells were transiently transfected in a 96-well plate using increasing amount (range: 0–200 ng/well) of β-arrestin 2-encoding pcDNA3.1 plasmid ( ) or commercial siRNA probes against β-arrestin 2 (#14387; Life-Technologies, Carlsbad, CA, USA) , using an electroporator (Gene Pulser MXcell; Bio-Rad Laboratories Inc., Hercules, CA, USA) in accordance with established protocol and settings ( , ).

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